Dissection of the Adult Drosophila CNS. This is a start-to-finish video showing the dissection of a fruit fly brain. The video begins by introducing the tools and materials and then goes through the process step by step. The method is suitable for downstream staining (eg immunohistochemistry or enzymatic) and is intended to be quick. Because the dissection is so quick, it can be (and is) performed in paraformaldehyde fixative - fixing two birds with one stone as it were. There is always a risk of distortion to the brain during dissection (by stretching) which is somewhat minimised by fixation. Most of the materials should be available in a basic laboratory - the most critical is very very sharp forceps (tweezers). The video shows a sharpening stone and you should sharpen your own tools for the best results. The video has been converted from an old 1993 VHS format with no soundtrack but a written protocol and associated example images have been on-line since the 90's at flybase.org which I believe makes it one of the earliest on-line protocols in bioscience. Comments welcomed even though I have moved up the evolutionary scale to work on arabidopsis now :) . .
Monday, January 21, 2013
fly93to09
fly93to09 Video Clips. Duration : 8.77 Mins.
Dissection of the Adult Drosophila CNS. This is a start-to-finish video showing the dissection of a fruit fly brain. The video begins by introducing the tools and materials and then goes through the process step by step. The method is suitable for downstream staining (eg immunohistochemistry or enzymatic) and is intended to be quick. Because the dissection is so quick, it can be (and is) performed in paraformaldehyde fixative - fixing two birds with one stone as it were. There is always a risk of distortion to the brain during dissection (by stretching) which is somewhat minimised by fixation. Most of the materials should be available in a basic laboratory - the most critical is very very sharp forceps (tweezers). The video shows a sharpening stone and you should sharpen your own tools for the best results. The video has been converted from an old 1993 VHS format with no soundtrack but a written protocol and associated example images have been on-line since the 90's at flybase.org which I believe makes it one of the earliest on-line protocols in bioscience. Comments welcomed even though I have moved up the evolutionary scale to work on arabidopsis now :) . .
Dissection of the Adult Drosophila CNS. This is a start-to-finish video showing the dissection of a fruit fly brain. The video begins by introducing the tools and materials and then goes through the process step by step. The method is suitable for downstream staining (eg immunohistochemistry or enzymatic) and is intended to be quick. Because the dissection is so quick, it can be (and is) performed in paraformaldehyde fixative - fixing two birds with one stone as it were. There is always a risk of distortion to the brain during dissection (by stretching) which is somewhat minimised by fixation. Most of the materials should be available in a basic laboratory - the most critical is very very sharp forceps (tweezers). The video shows a sharpening stone and you should sharpen your own tools for the best results. The video has been converted from an old 1993 VHS format with no soundtrack but a written protocol and associated example images have been on-line since the 90's at flybase.org which I believe makes it one of the earliest on-line protocols in bioscience. Comments welcomed even though I have moved up the evolutionary scale to work on arabidopsis now :) . .
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